Nitrogen Source Dependent Changes in Central Sugar Metabolism Maintain Cell Wall Assembly in Mitochondrial Complex I-Defective frostbite1 and Secondarily Affect Programmed Cell Death.
Int J Mol Sci
; 19(8)2018 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-30060552
ABSTRACT
For optimal plant growth, carbon and nitrogen availability needs to be tightly coordinated. Mitochondrial perturbations related to a defect in complex I in the Arabidopsis thalianafrostbite1 (fro1) mutant, carrying a point mutation in the 8-kD Fe-S subunit of NDUFS4 protein, alter aspects of fundamental carbon metabolism, which is manifested as stunted growth. During nitrate nutrition, fro1 plants showed a dominant sugar flux toward nitrogen assimilation and energy production, whereas cellulose integration in the cell wall was restricted. However, when cultured on NH4⺠as the sole nitrogen source, which typically induces developmental disorders in plants (i.e., the ammonium toxicity syndrome), fro1 showed improved growth as compared to NO3- nourishing. Higher energy availability in fro1 plants was correlated with restored cell wall assembly during NH4⺠growth. To determine the relationship between mitochondrial complex I disassembly and cell wall-related processes, aspects of cell wall integrity and sugar and reactive oxygen species signaling were analyzed in fro1 plants. The responses of fro1 plants to NH4⺠treatment were consistent with the inhibition of a form of programmed cell death. Resistance of fro1 plants to NH4⺠toxicity coincided with an absence of necrotic lesion in plant leaves.
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MEDLINE
Assunto principal:
Parede Celular
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Arabidopsis
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Proteínas de Arabidopsis
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Açúcares
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NADH Desidrogenase
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Nitrogênio
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article