Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis.
Clin Cancer Res
; 24(22): 5635-5644, 2018 11 15.
Article
em En
| MEDLINE
| ID: mdl-30093450
ABSTRACT
Purpose:
Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency.ExperimentalDesign:
We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity.Results:
Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses.Conclusions:
Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635-44. ©2018 AACR.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Remoção de Componentes Sanguíneos
/
Biomarcadores Tumorais
/
Análise de Célula Única
/
Biópsia Líquida
Tipo de estudo:
Prognostic_studies
Limite:
Humans
/
Male
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article