Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types.
Mem Inst Oswaldo Cruz
; 113(10): e180340, 2018.
Article
em En
| MEDLINE
| ID: mdl-30231112
ABSTRACT
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). This study aimed to optimise single-step multiplex PCR for the accurate detection of the distinct mating types of H. capsulatum. Among the 26 isolates tested, 20 had MAT1-1 genotype, while six showed MAT1-2 genotype, in agreement with the uniplex PCR results. These results suggest that multiplex PCR is a fast and specific tool for screening H. capsulatum mating types.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Fúngico
/
Primers do DNA
/
Histoplasma
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
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Screening_studies
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article