One-Step Chemical Synthesis of Native Met1-Linked Poly-Ubiquitin Chains.
Chembiochem
; 20(1): 62-65, 2019 01 02.
Article
em En
| MEDLINE
| ID: mdl-30264452
ABSTRACT
The enzyme-mediated construction of poly-ubiquitin (Ub) chains on target proteins leads to a variety of cellular responses and is involved in processes ranging from protein degradation to cell cycle control and immune responses. This complex post-translational modification system is under intense investigation, but generation of specific Ub chains and tools made thereof is not always trivial. We discovered that native methionine-1-linked polymeric ubiquitin chains can be constructed in a single chemical reaction. We validate correct folding and regioselective attachment of such chains using linkage specific proteases and further demonstrate that these poly-Ub chains can be converted into thioesters by the activating E1-enzyme. Subsequent ligation reactions using these in situ prepared thioesters leads to poly-ubiquitinated peptides.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poliubiquitina
/
Metionina
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article