bioNMR-based identification of natural anti-Aß compounds in Peucedanum ostruthium.
Bioorg Chem
; 83: 76-86, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30342388
ABSTRACT
The growing interest in medicinal plants for the identification of new bioactive compounds and the formulation of new nutraceuticals and drugs prompted us to develop a powerful experimental approach allowing the detailed metabolic profiling of complex plant extracts, the identification of ligands of macromolecular targets of biomedical relevance and a preliminary characterization of their biological activity. To this end, we selected Peucedanum ostruthium, a plant traditionally employed in Austria and Italy for its several potential therapeutic applications, as case study. We combined the use of NMR and UPLC-HR-MS for the identification of the metabolites present in its leaves and rhizome extracts. Due to the significant content of polyphenols, particularly chlorogenic acids, recently identified as anti-amyloidogenic compounds, polyphenols-enriched fractions were prepared and tested for their ability to prevent Aß1-42 peptide aggregation and neurotoxicity in a neuronal human cell line. STD-NMR experiments allowed the detailed identification of Aß oligomers' ligands responsible for the anti-amyloidogenic activity. These data provide experimental protocols and structural information suitable for the development of innovative molecular tools for prevention, therapy and diagnosis of Alzheimer's disease.
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Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
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Extratos Vegetais
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Peptídeos beta-Amiloides
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Apiaceae
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Ressonância Magnética Nuclear Biomolecular
Tipo de estudo:
Diagnostic_studies
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Guideline
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article