Synthesis of antithrombin III and alpha-1-antitrypsin by the perfused rat liver.
Biochim Biophys Acta
; 539(4): 496-504, 1978 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-305791
ABSTRACT
Livers isolated from control or turpentine-injected rats were perfused for 3 h with human red cells suspended in Krebs-Henseleit solution containing bovine serum albumin, dextran, glucose, heparin, cortisol, insulin, a mixture of 20 amino acids and [3H]leucine. Changes in the concentrations of antithrombin III and alpha-1-antitrypsin were evaluated by rocket immunoelectrophoresis using specific antisera, and incorporation of the 3H radioactivity into the total protein, albumin, antitrhombin III and alpha-1-antitrypsin in the perfusate was measured. The results indicate that both antithrombin III and alpha-1-antitrypsin are synthesized in the liver. Local inflammation induced in the liver donors moderately stimulated the synthesis of alpha-1-antitrypsin but it affected only marginally that of antithrombin III.
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Base de dados:
MEDLINE
Assunto principal:
Antitrombinas
/
Alfa 1-Antitripsina
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article