Your browser doesn't support javascript.
loading
Vasoactive intestinal peptide is required in the maintenance of immune regulatory competency of immune regulatory monocytes.
Guan, L; Yu, D; Wu, G-H; Ning, H-J; He, S-D; Li, S-S; Hu, T-Y; Yang, G; Liu, Z-Q; Yu, H-Q; Sun, X-Z; Liu, Z-G; Yang, P-C.
Afiliação
  • Guan L; Department of Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
  • Yu D; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Wu GH; Department of Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
  • Ning HJ; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • He SD; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Li SS; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Hu TY; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Yang G; Longgang ENT Hospital and Shenzhen ENT Institute, Shenzhen, China.
  • Liu ZQ; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Yu HQ; Longgang ENT Hospital and Shenzhen ENT Institute, Shenzhen, China.
  • Sun XZ; Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen, China.
  • Liu ZG; Longgang ENT Hospital and Shenzhen ENT Institute, Shenzhen, China.
  • Yang PC; Department of Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
Clin Exp Immunol ; 196(2): 276-286, 2019 05.
Article em En | MEDLINE | ID: mdl-30636174
ABSTRACT
Dysfunction of the immune regulatory system plays an important role in the pathogenesis of rheumatoid arthritis (RA). Vasoactive intestinal peptide (VIP) has multiple bioactivities. This study aims to investigate the role of VIP in the maintenance of the immune regulatory capacity of monocytes (Mos). Human peripheral blood samples were collected from RA patients and healthy control (HC) subjects. Mos and CD14+ CD71- CD73+ CD25+ regulatory Mos (RegMos) were isolated from the blood samples and characterized by flow cytometry. A rat RA model was developed to test the role of VIP in the maintenance of the immune regulatory function of Mos. The results showed that RegMos of HC subjects had immune suppressive functions. RegMos of RA patients expressed less interleukin (IL)-10 and showed an incompetent immune regulatory capacity. Serum levels of VIP were lower in RA patients, which were positively correlated with the expression of IL-10 in RegMos. In-vitro experiments showed that the IL-10 mRNA decayed spontaneously in RegMos, which could be prevented by the presence of VIP in the culture. VIP suppressed the effects of tristetraprolin (TTP) on inducing IL-10 mRNA decay in RegMos. Administration of VIP inhibited experimental RA in rats through restoring the IL-10 expression in RegMos. RegMos have immune suppressive functions. VIP is required in maintaining IL-10 expression in RegMos. The data suggest that VIP has translational potential in the treatment of immune disorders such as RA.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Intestinal Vasoativo / Monócitos Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Intestinal Vasoativo / Monócitos Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans / Male Idioma: En Ano de publicação: 2019 Tipo de documento: Article