5-Methylcytosine-Rich Heterochromatin in Reptiles.
Cytogenet Genome Res
; 157(1-2): 53-64, 2019.
Article
em En
| MEDLINE
| ID: mdl-30641524
ABSTRACT
An experimental approach using monoclonal anti-5-methylcytosine antibodies and indirect immunofluorescence was elaborated for detecting 5-methylcytosine-rich chromosome regions in reptilian chromosomes. This technique was applied to conventionally prepared mitotic metaphases of 2 turtle species and 12 squamate species from 8 families. The hypermethylation patterns were compared with C-banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and are located in constitutive heterochromatin. They are highly reproducible and often found in centromeric, pericentromeric, and interstitial positions of the chromosomes. Heterochromatic regions in differentiated sex chromosomes are particularly hypermethylated.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Répteis
/
Heterocromatina
/
Cromossomos
/
5-Metilcitosina
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article