Rumen-derived lipopolysaccharide induced ruminal epithelium barrier damage in goats fed a high-concentrate diet.
Microb Pathog
; 131: 81-86, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30910720
ABSTRACT
This study aimed to investigate the mechanism of lipopolysaccharide (LPS) released in the rumen on epithelium barrier function of goats fed a HC diet. Twelve Boer goats were randomly divided into two groups low-concentrate(LC) diet and high-concentrate(HC) diet treatment. We found that the pH of rumen fluid in the HC group was lower than in the LC group (Pâ¯<â¯0.05). The mRNA and protein expression levels of p38 mitogen-activated protein kinase (MAPK), extracellular regulated protein kinases (ERK), and c-Jun N-terminal kinase (JNK) in the rumen epithelium were lower in the LC group than the HC group (Pâ¯<â¯0.05). Gene expression and protein levels of the tight junction proteins claudin-1, claudin-4, occludin, and Zona occludin-1 were all greater in the LC group than the HC group (Pâ¯<â¯0.05). Staining of claudin-1, occludin and ZO-1 was became irregular. In conclusion, high concentrate diet feeding can impair rumen epithelium function and decrease tight junction protein expression through MAPK signaling pathway.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Rúmen
/
Lipopolissacarídeos
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Dieta
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Epitélio
Limite:
Animals
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article