Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples - a proof-of-principle assessment.

Frickmann, Hagen; Künne, Carsten; Hagen, Ralf Matthias; Podbielski, Andreas; Normann, Jana; Poppert, Sven; Looso, Mario; Kreikemeyer, Bernd

Frickmann, Hagen; Künne, Carsten; Hagen, Ralf Matthias; Podbielski, Andreas; Normann, Jana; Poppert, Sven; Looso, Mario; Kreikemeyer, Bernd.

Afiliação

Frickmann H; Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, Bernhard-Nocht Str. 74, 20359, Hamburg, Germany. Frickmann@bnitm.de.
Künne C; Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057, Rostock, Germany. Frickmann@bnitm.de.
Hagen RM; Department of Bioinformatics, Max-Planck Institute for Heart and Lung Research Bad Nauheim, Parkstraße 1, 61231, Bad Nauheim, Germany.
Podbielski A; Department of Preventive Medicine, Bundeswehr Medical Academy, Neuherbergstraße 11, 80937, Munich, Germany.
Normann J; Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057, Rostock, Germany.
Poppert S; Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057, Rostock, Germany.
Looso M; Swiss Tropical and Public Health Institute, Socinstrasse 57, 4051, Basel, Switzerland.
Kreikemeyer B; Faculty of Medicine, University Basel, Socinstrasse 57, 4051, Basel, Switzerland.

BMC Microbiol ; 19(1): 75, 2019 04 08.

Article em En | MEDLINE | ID: mdl-30961537

Assuntos

Amebíase/diagnóstico; Coinfecção/microbiologia; Sequenciamento de Nucleotídeos em Larga Escala; Infecções Fúngicas Invasivas/diagnóstico; Coinfecção/diagnóstico; Entamoeba histolytica/genética; Entamoeba histolytica/patogenicidade; Formaldeído; Fungos/genética; Fungos/patogenicidade; Genômica; Humanos; Infecções Fúngicas Invasivas/microbiologia; Inclusão em Parafina; Estudo de Prova de Conceito; Análise de Sequência de DNA; Fixação de Tecidos

Palavras-chave

FFPE, formalin-fixed, paraffin-embedded samples; Hypothesis-free diagnosis of infection; Invasive amebiasis; Invasive fungal infections; Metagenome; Molecular diagnostics; NGS; Next-generation sequencing; Tropical infectious diseases

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sequenciamento de Nucleotídeos em Larga Escala / Coinfecção / Infecções Fúngicas Invasivas / Amebíase Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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