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The immune cell landscape in kidneys of patients with lupus nephritis.
Arazi, Arnon; Rao, Deepak A; Berthier, Celine C; Davidson, Anne; Liu, Yanyan; Hoover, Paul J; Chicoine, Adam; Eisenhaure, Thomas M; Jonsson, A Helena; Li, Shuqiang; Lieb, David J; Zhang, Fan; Slowikowski, Kamil; Browne, Edward P; Noma, Akiko; Sutherby, Danielle; Steelman, Scott; Smilek, Dawn E; Tosta, Patti; Apruzzese, William; Massarotti, Elena; Dall'Era, Maria; Park, Meyeon; Kamen, Diane L; Furie, Richard A; Payan-Schober, Fernanda; Pendergraft, William F; McInnis, Elizabeth A; Buyon, Jill P; Petri, Michelle A; Putterman, Chaim; Kalunian, Kenneth C; Woodle, E Steve; Lederer, James A; Hildeman, David A; Nusbaum, Chad; Raychaudhuri, Soumya; Kretzler, Matthias; Anolik, Jennifer H; Brenner, Michael B; Wofsy, David; Hacohen, Nir; Diamond, Betty.
Afiliação
  • Arazi A; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Rao DA; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Berthier CC; Internal Medicine, Department of Nephrology, University of Michigan, Ann Arbor, MI, USA.
  • Davidson A; Center for Autoimmune and Musculoskeletal Diseases, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, USA.
  • Liu Y; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Hoover PJ; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Chicoine A; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Eisenhaure TM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Jonsson AH; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Li S; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Lieb DJ; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Zhang F; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Slowikowski K; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Browne EP; UNC HIV Cure Center and Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Noma A; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Sutherby D; Celsius Therapeutics, Cambridge, MA, USA.
  • Steelman S; Cellarity, Inc., Cambridge, MA, USA.
  • Smilek DE; Lupus Nephritis Trials Network, University of California San Francisco, San Francisco, CA, USA.
  • Tosta P; Immune Tolerance Network, University of California San Francisco, San Francisco, CA, USA.
  • Apruzzese W; Lupus Nephritis Trials Network, University of California San Francisco, San Francisco, CA, USA.
  • Massarotti E; Immune Tolerance Network, University of California San Francisco, San Francisco, CA, USA.
  • Dall'Era M; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Park M; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Kamen DL; Rheumatology Division, University of California San Francisco, San Francisco, CA, USA.
  • Furie RA; Division of Nephrology, University of California San Francisco, San Francisco, CA, USA.
  • Payan-Schober F; Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, SC, USA.
  • Pendergraft WF; Division of Rheumatology, Northwell Health, Great Neck, NY, USA.
  • McInnis EA; Department of Medicine, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX, USA.
  • Buyon JP; The Integrative Medical Clinic of North Carolina, PLLC, Chapel Hill, NC, USA.
  • Petri MA; University of North Carolina Kidney Center, Division of Nephrology and Hypertension, Department of Medicine, UNC School of Medicine, Chapel Hill, NC, USA.
  • Putterman C; Department of Medicine, Division of Rheumatology, New York University School of Medicine, New York, NY, USA.
  • Kalunian KC; Division of Rheumatology, Johns Hopkins University, Baltimore, MD, USA.
  • Woodle ES; Division of Rheumatology and Department of Microbiology and Immunology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA.
  • Lederer JA; University of California San Diego School of Medicine, La Jolla, CA, USA.
  • Hildeman DA; Division of Transplantation, Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
  • Nusbaum C; Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Raychaudhuri S; Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA.
  • Kretzler M; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
  • Anolik JH; Cellarity, Inc., Cambridge, MA, USA.
  • Brenner MB; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Wofsy D; Internal Medicine, Department of Nephrology, University of Michigan, Ann Arbor, MI, USA.
  • Hacohen N; Department of Medicine, Division of Allergy, Immunology, and Rheumatology, University of Rochester Medical Center, Rochester, NY, USA.
  • Diamond B; Division of Rheumatology, Immunology, Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Nat Immunol ; 20(7): 902-914, 2019 07.
Article em En | MEDLINE | ID: mdl-31209404
ABSTRACT
Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid cells, T cells, natural killer cells and B cells that demonstrated both pro-inflammatory responses and inflammation-resolving responses. We found evidence of local activation of B cells correlated with an age-associated B-cell signature and evidence of progressive stages of monocyte differentiation within the kidney. A clear interferon response was observed in most cells. Two chemokine receptors, CXCR4 and CX3CR1, were broadly expressed, implying a potentially central role in cell trafficking. Gene expression of immune cells in urine and kidney was highly correlated, which would suggest that urine might serve as a surrogate for kidney biopsies.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nefrite Lúpica / Rim Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nefrite Lúpica / Rim Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article