Synthetic Simplification of Carolacton Enables Chemical Genetic Studies in Streptococcus mutans.
ACS Infect Dis
; 5(8): 1480-1486, 2019 08 09.
Article
em En
| MEDLINE
| ID: mdl-31243986
ABSTRACT
Understanding the broader biological impact of carolacton, a macrolactone natural product, has been ongoing for the past decade. Multiple studies have shown connections to regulatory systems, acid tolerance mechanisms, biofilm formation, and recently folate dehydrogenase (FolD). Progress elucidating the cause of biofilm-specific activity in Streptococcus mutans has been limited due to low-throughput analyses of carolacton-treated cells. We disclose the discovery of a simplified carolacton-inspired analog that demonstrates inhibitory activity against S. mutans biofilm cells. This discovery permitted a proof of concept chemical genetic screen of S. mutans mutants identifying the carbon catabolite protein A signaling pathway as a putative target.
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Base de dados:
MEDLINE
Assunto principal:
Streptococcus mutans
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Biofilmes
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Macrolídeos
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article