Highly sensitive and specific LC-MS/MS method to determine endogenous leukotriene B4 levels in human plasma.
Bioanalysis
; 11(11): 1055-1066, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-31251101
ABSTRACT
Aim:
To develop a high sensitivity and specific analytical method to measure endogenous levels of leukotriene B4 (LTB4) in human plasma.Methodology:
LC-MS/MS and ELISA.Results:
An LC-MS/MS method was developed with a sensitivity of 1.0 pg/ml, and within and between batch precision of <16% and <13% RSD, respectively.Conclusion:
We have developed a sensitive LC-MS/MS method that can detect endogenous LTB4 in human plasma. The LC-MS/MS method displayed correlation with a commercial LTB4 ELISA when analyzing in ex vivo ionophore-stimulated blood samples. For untreated plasma this correlation was lost. Endogenous LTB4 was shown to be unstable in plasma during storage at -20°C and subject to stereoisomer formation. Neither of the assays could quantify endogenous plasma LTB4 in samples stored for long term.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leucotrieno B4
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article