Production and characterization of low-calorie turanose and digestion-resistant starch by an amylosucrase from Neisseria subflava.
Food Chem
; 300: 125225, 2019 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-31351257
ABSTRACT
This study was intended to produce turanose and resistant starch (RS) using recombinant amylosucrase from Neisseria subflava (NsAS). Turanose production yield maximally reached to 76% of sucrose substrate at 40⯰C by NsAS treatment. To evaluate turanose as a low-calorie functional sweetener, its hydrolysis pattern was investigated in continuous artificial digestion system. When turanose was consecutively exposed through small intestinal phase, only 8% of disaccharide was hydrolyzed. Structural modification of gelatinized corn or rice starch was carried out by NsAS with sucrose as a glucosyl donor. Non-digestibility of enzyme-modified starches increased to 47.3% maximally through branch-chain elongation, enough for chain-chain association and recrystallization. Obviously, NsAS-modified starches had higher gelatinization peak temperatures than native counterparts, and their paste viscosity was inversely related to their digestibility due to elongated-chain induced retrogradation. These results suggested that NsAS could be a vital biocatalyst candidate in food industry to produce next generation low-calorie carbohydrate food materials.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Amido
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Dissacarídeos
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Glucosiltransferases
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Neisseria
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article