Covalently tethering siRNA to hydrogels for localized, controlled release and gene silencing.
Sci Adv
; 5(8): eaax0801, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31489374
ABSTRACT
Small interfering RNA (siRNA) has found many applications in tissue regeneration and disease therapeutics. Effective and localized siRNA delivery remains challenging, reducing its therapeutic potential. Here, we report a strategy to control and prolong siRNA release by directly tethering transfection-capable siRNA to photocrosslinked dextran hydrogels. siRNA release is governed via the hydrolytic degradation of ester and/or disulfide linkages between the siRNA and hydrogels, which is independent of hydrogel degradation rate. The released siRNA is shown to be bioactive by inhibiting protein expression in green fluorescent protein-expressing HeLa cells without the need of a transfection agent. This strategy provides an excellent platform for controlling nucleic acid delivery through covalent bonds with a biomaterial and regulating cellular gene expression, which has promising potential in many biomedical applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hidrogéis
/
Inativação Gênica
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Preparações de Ação Retardada
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RNA Interferente Pequeno
Limite:
Humans
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article