Characterization of a novel Clostridium sp. SP17-B1 and its application for succinic acid production from hevea wood waste hydrolysate.
Anaerobe
; 61: 102096, 2020 Feb.
Article
em En
| MEDLINE
| ID: mdl-31493499
ABSTRACT
An anaerobic, gram-positive, rod-shaped bacterium strain SP17-B1, isolated from dog saliva, was taxonomically characterized on the basis of phenotypic, chemotaxonomic, and genotypic characteristics. It was cultured in 4% (w/v) NaCl at a pH range of 5.0-8.0 (optimally at pH 7) and at 30°C-40⯰C (optimally at 37⯰C). Its major cellular fatty acids are C160 (36.3%), C170 cyclo (9.7%), C161ω9c (13.9%), and C181ω9c (10.7%), and its DNA guanine-cytosine content is 40.8â¯mol%. On the basis of the 16S rRNA gene sequence analysis, it was determined that the strain belonged to the genus Clostridium and was closely related to C. amygdalinum BR-10T (97.8%), C. saccharolyticum WM1T (97.8%), and C. celleracrescens DSM 5628T (97.7%). This strain showed a low level of DNA-DNA relatedness with the closely related strains, suggesting that it is a novel species in the genus Clostridium. Recent studies have demonstrated the production of succinic acid using Clostridium strains. Strain SP17-B1 produced 25.1⯱â¯1.3 and 15.3⯱â¯1.5â¯g/L of succinic acid from 40â¯g/L of glucose and 30â¯g/L of hevea wood waste hydrolysate (HH), respectively, after 24â¯h. When detoxified HH was used as a substrate, the lag phase was reduced and cell growth was enhanced by 7 fold (OD660 0.4-3.0) within 12â¯h. Detoxification using granular activated carbon may have reduced the levels of furfural and HMF without interfering with the amount of sugars in HH.
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MEDLINE
Assunto principal:
Resíduos
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Madeira
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Clostridium
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Ácido Succínico
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Hevea
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Fermentação
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article