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Long-Distance Cooperative and Antagonistic RNA Polymerase Dynamics via DNA Supercoiling.
Kim, Sangjin; Beltran, Bruno; Irnov, Irnov; Jacobs-Wagner, Christine.
Afiliação
  • Kim S; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA. Electronic address: sangjin@illinois.edu.
  • Beltran B; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA.
  • Irnov I; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA.
  • Jacobs-Wagner C; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA; Department of Microbial Pathogenesis, Yale Sch
Cell ; 179(1): 106-119.e16, 2019 Sep 19.
Article em En | MEDLINE | ID: mdl-31539491
ABSTRACT
Genes are often transcribed by multiple RNA polymerases (RNAPs) at densities that can vary widely across genes and environmental conditions. Here, we provide in vitro and in vivo evidence for a built-in mechanism by which co-transcribing RNAPs display either collaborative or antagonistic dynamics over long distances (>2 kb) through transcription-induced DNA supercoiling. In Escherichia coli, when the promoter is active, co-transcribing RNAPs translocate faster than a single RNAP, but their average speed is not altered by large variations in promoter strength and thus RNAP density. Environmentally induced promoter repression reduces the elongation efficiency of already-loaded RNAPs, causing premature termination and quick synthesis arrest of no-longer-needed proteins. This negative effect appears independent of RNAP convoy formation and is abrogated by topoisomerase I activity. Antagonistic dynamics can also occur between RNAPs from divergently transcribed gene pairs. Our findings may be broadly applicable given that transcription on topologically constrained DNA is the norm across organisms.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transcrição Gênica / RNA Polimerases Dirigidas por DNA / DNA Bacteriano / DNA Super-Helicoidal / Escherichia coli Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transcrição Gênica / RNA Polimerases Dirigidas por DNA / DNA Bacteriano / DNA Super-Helicoidal / Escherichia coli Idioma: En Ano de publicação: 2019 Tipo de documento: Article