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Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells.
Palacios-Moreno, Juan; Rubio, Cecilia; Quilhot, Wanda; Cavieres, M Fernanda; de la Peña, Eduardo; Quiñones, Natalia V; Díaz, Hugo; Carrión, Flavio; Henríquez-Roldán, Carlos F; Weinstein-Oppenheimer, Caroline R.
Afiliação
  • Palacios-Moreno J; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, CP 2360102, Valparaiso, Chile.
  • Rubio C; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, CP 2360102, Valparaiso, Chile.
  • Quilhot W; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, CP 2360102, Valparaiso, Chile.
  • Cavieres MF; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, CP 2360102, Valparaiso, Chile.
  • de la Peña E; Centro de Investigación Farmacopea Chilena, Valparaiso, Chile.
  • Quiñones NV; ICA-Mutagénesis Ambiental, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
  • Díaz H; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Av. Gran Bretaña 1093, Playa Ancha, CP 2360102, Valparaiso, Chile.
  • Carrión F; Centro de Investigación Farmacopea Chilena, Valparaiso, Chile.
  • Henríquez-Roldán CF; Escuela de Ingeniería en Medioambiente, Facultad de Ingeniería, Universidad de Valparaíso, Valparaiso, Chile.
  • Weinstein-Oppenheimer CR; Programa de Inmunología Traslacional, Facultad de Medicina, Clínica Alemana, Universidad del Desarrollo, Las Condes, Santiago, Chile.
Biol Res ; 52(1): 55, 2019 Oct 10.
Article em En | MEDLINE | ID: mdl-31601259
ABSTRACT

BACKGROUND:

Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay.

RESULTS:

MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP's mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP's lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP.

CONCLUSION:

EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Apoptose / Proliferação de Células / Líquens / Antineoplásicos Limite: Female / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Apoptose / Proliferação de Células / Líquens / Antineoplásicos Limite: Female / Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article