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Adjuvants Enhance the Induction of Germinal Center and Antibody Secreting Cells in Spleen and Their Persistence in Bone Marrow of Neonatal Mice.
Aradottir Pind, Audur Anna; Dubik, Magdalena; Thorsdottir, Sigrun; Meinke, Andreas; Harandi, Ali M; Holmgren, Jan; Del Giudice, Giuseppe; Jonsdottir, Ingileif; Bjarnarson, Stefania P.
Afiliação
  • Aradottir Pind AA; Department of Immunology, Landspitali, The National University Hospital of Iceland, Reykjavik, Iceland.
  • Dubik M; Faculty of Medicine, School of Health Sciences, University of Iceland, Reykjavik, Iceland.
  • Thorsdottir S; Department of Immunology, Landspitali, The National University Hospital of Iceland, Reykjavik, Iceland.
  • Meinke A; Faculty of Medicine, School of Health Sciences, University of Iceland, Reykjavik, Iceland.
  • Harandi AM; Department of Immunology, Landspitali, The National University Hospital of Iceland, Reykjavik, Iceland.
  • Holmgren J; Valneva Austria GmbH, Vienna, Austria.
  • Del Giudice G; Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
  • Jonsdottir I; Vaccine Evaluation Center, BC Children's Hospital Research Institute, The University of British Columbia, Vancouver, BC, Canada.
  • Bjarnarson SP; Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Front Immunol ; 10: 2214, 2019.
Article em En | MEDLINE | ID: mdl-31616417
Immaturity of the immune system contributes to poor vaccine responses in early life. Germinal center (GC) activation is limited due to poorly developed follicular dendritic cells (FDC), causing generation of few antibody-secreting cells (ASCs) with limited survival and transient antibody responses. Herein, we compared the potential of five adjuvants, namely LT-K63, mmCT, MF59, IC31, and alum to overcome limitations of the neonatal immune system and to enhance and prolong responses of neonatal mice to a pneumococcal conjugate vaccine Pnc1-TT. The adjuvants LT-K63, mmCT, MF59, and IC31 significantly enhanced GC formation and FDC maturation in neonatal mice when co-administered with Pnc1-TT. This enhanced GC induction correlated with significantly enhanced vaccine-specific ASCs by LT-K63, mmCT, and MF59 in spleen 14 days after immunization. Furthermore, mmCT, MF59, and IC31 prolonged the induction of vaccine-specific ASCs in spleen and increased their persistence in bone marrow up to 9 weeks after immunization, as previously shown for LT-K63. Accordingly, serum Abs persisted above protective levels against pneumococcal bacteremia and pneumonia. In contrast, alum only enhanced the primary induction of vaccine-specific IgG Abs, which was transient. Our comparative study demonstrated that, in contrast to alum, LT-K63, mmCT, MF59, and IC31 can overcome limitations of the neonatal immune system and enhance both induction and persistence of protective immune response when administered with Pnc1-TT. These adjuvants are promising candidates for early life vaccination.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Baço / Medula Óssea / Adjuvantes Imunológicos / Centro Germinativo / Células Produtoras de Anticorpos Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Baço / Medula Óssea / Adjuvantes Imunológicos / Centro Germinativo / Células Produtoras de Anticorpos Limite: Animals Idioma: En Ano de publicação: 2019 Tipo de documento: Article