A genetically encoded fluorescent biosensor for detecting nitroreductase activity in living cancer cells.

Xu, Fangyuan; Fan, Mengyue; Kang, Saisai; Duan, Xinrui

Xu, Fangyuan; Fan, Mengyue; Kang, Saisai; Duan, Xinrui.

Afiliação

Xu F; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and School of Chemistry and Chemical Engineering, Shaanxi Normal University, 620 Xi Chang'an Street, Xi'an, Shaanxi, 710119, PR China.
Fan M; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and School of Chemistry and Chemical Engineering, Shaanxi Normal University, 620 Xi Chang'an Street, Xi'an, Shaanxi, 710119, PR China.
Kang S; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and School of Chemistry and Chemical Engineering, Shaanxi Normal University, 620 Xi Chang'an Street, Xi'an, Shaanxi, 710119, PR China.
Duan X; Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and School of Chemistry and Chemical Engineering, Shaanxi Normal University, 620 Xi Chang'an Street, Xi'an, Shaanxi, 710119, PR China. Electronic address: duanxr@snnu.edu.cn.

Anal Chim Acta ; 1088: 131-136, 2019 Dec 11.

Article em En | MEDLINE | ID: mdl-31623708

Assuntos

Técnicas Biossensoriais/métodos; Corantes Fluorescentes/metabolismo; Nitrorredutases/metabolismo; Sobrevivência Celular; Proteínas de Fluorescência Verde/genética; Proteínas de Fluorescência Verde/metabolismo; Células HeLa; Humanos; Microscopia Confocal; RNA/metabolismo

Palavras-chave

Cell imaging; Nitroreductase; RNA aptamer; RNA mimics of GFP

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nitrorredutases / Técnicas Biossensoriais / Corantes Fluorescentes Limite: Humans Idioma: En Ano de publicação: 2019 Tipo de documento: Article

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