Genetic diversity of the Metarhizium anisopliae complex in Colima, Mexico, using microsatellites.
Fungal Biol
; 123(12): 855-863, 2019 12.
Article
em En
| MEDLINE
| ID: mdl-31733728
ABSTRACT
Metarhizium anisopliae is a complex of cryptic species with wide geographical distribution and versatile lifestyles. In this study, 45 isolates of the Metarhizium genus harbored in the "Colección de Hongos Entomopatógenos" of the "Centro Nacional de Referencia de Control Biológico" from different substrates, insect-host, and localities from Colima, Mexico, were phylogenetically identified using the 5'end of translation elongation factor 1-α (5'TEF) and intergenic nuclear region MzFG543igs. Seven species were recognized, M. acridum (n = 26), M. pemphigi (n = 1), and within the PARB and MGT clades M. anisopliae (N = 7; sensu stricto n = 2; sensu lato n = 5), M. brunneum (n = 2), M. guizhouense (n = 2), M. pingshaense (n = 2), and M. robertsii (n = 5). Twenty-nine SSR markers were developed for M. acridum; according to the analysis of 12 polymorphic SSR loci, M. acridum showed low genetic diversity, revealing five genotypes with a dominant one (n = 21). Based on the analysis of 13 specific SSR loci, 14 genotypes were identified within the PARB and MGT clades. This study contributes to generating valuable information about the community structure and genotypic diversity of Metharhizum species in the state of Colima, Mexico.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Variação Genética
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DNA Fúngico
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Repetições de Microssatélites
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Metarhizium
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Genótipo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Mexico
Idioma:
En
Ano de publicação:
2019
Tipo de documento:
Article