Characterizing the role of Zn cluster family transcription factor ZcfA in governing development in two Aspergillus species.
PLoS One
; 15(2): e0228643, 2020.
Article
em En
| MEDLINE
| ID: mdl-32017793
ABSTRACT
Filamentous fungi reproduce asexually or sexually, and the processes of asexual and sexual development are tightly regulated by a variety of transcription factors. In this study, we characterized a Zn2Cys6 transcription factor in two Aspergillus species, A. nidulans (AN5859) and A. flavus (AFLA_046870). AN5859 encodes a Zn2Cys6 transcription factor, called ZcfA. In A. nidulans, ΔzcfA mutants exhibit decreased fungal growth, a reduction in cleistothecia production, and increased asexual reproduction. Overexpression of zcfA results in increased conidial production, suggesting that ZcfA is required for proper asexual and sexual development in A. nidulans. In conidia, deletion of zcfA causes decreased trehalose levels and decreased spore viability but increased thermal sensitivity. In A. flavus, the deletion of the zcfA homolog AFLA_046870 causes increased conidial production but decreased sclerotia production; these effects are similar to those of zcfA deletion in A. nidulans development. Overall, these results demonstrate that ZcfA is essential for maintaining a balance between asexual and sexual development and that some roles of ZcfA are conserved in Aspergillus spp.
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Base de dados:
MEDLINE
Assunto principal:
Aspergillus flavus
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Aspergillus nidulans
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Fatores de Transcrição
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article