Structure-Mediated RNA Decay by UPF1 and G3BP1.
Mol Cell
; 78(1): 70-84.e6, 2020 04 02.
Article
em En
| MEDLINE
| ID: mdl-32017897
ABSTRACT
Post-transcriptional mechanisms regulate the stability and, hence, expression of coding and noncoding RNAs. Sequence-specific features within the 3' untranslated region (3' UTR) often direct mRNAs for decay. Here, we characterize a genome-wide RNA decay pathway that reduces the half-lives of mRNAs based on overall 3' UTR structure formed by base pairing. The decay pathway is independent of specific single-stranded sequences, as regulation is maintained in both the original and reverse complement orientation. Regulation can be compromised by reducing the overall structure by fusing the 3' UTR with an unstructured sequence. Mutating base-paired RNA regions can also compromise this structure-mediated regulation, which can be restored by re-introducing base-paired structures of different sequences. The decay pathway requires the RNA-binding protein UPF1 and its associated protein G3BP1. Depletion of either protein increased steady-state levels of mRNAs with highly structured 3' UTRs as well as highly structured circular RNAs. This structure-dependent mechanism therefore enables cells to selectively regulate coding and noncoding RNAs.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Transativadores
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DNA Helicases
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RNA Helicases
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Regiões 3' não Traduzidas
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Estabilidade de RNA
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Proteínas com Motivo de Reconhecimento de RNA
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Proteínas de Ligação a Poli-ADP-Ribose
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article