Bioaccumulation kinetics and tissue distribution of silver nanoparticles in zebrafish: The mechanisms and influence of natural organic matter.

ABSTRACT

The wide application of silver nanoparticles (AgNPs) has inevitably led to their release into the natural aquatic environment. Natural organic matter (NOM) is ubiquitous and would influence the fate and effects of these nanoparticles in such aquatic environments. Here we demonstrate that NOM plays an important role in the bioaccumulation kinetics and tissue distribution of AgNPs in zebrafish. In the presence of humic acid and fulvic acid, the uptake rates of AgNPs decreased while the depuration rates of AgNPs increased. As a result, the bioconcentration factor (BCF) of AgNPs in the entire body of the zebrafish was reduced. AgNPs were mainly taken up by the zebrafish via oral ingestion and were greatly accumulated in the liver, intestine and gill. In the intestine, NOM effectively inhibited the AgNPs from penetrating the cell membranes into internal tissues and also suppressed the disintegration and dissolution of AgNPs in gastrointestinal fluid, thereby decreasing the absorption of Ag by zebrafish. This research underlines the significance of incorporating the effects of NOM into predictive models for accurately assessing the toxicity and ecological risks of nanoparticles in natural aquatic environments.