High-Throughput Sequencing (HTS) of newly synthetized RNAs enables one shot detection and identification of live mycoplasmas and differentiation from inert nucleic acids.

Desbrousses, Céline; Archer, Fabienne; Colin, Adélie; Bobet-Erny, Alexandra; Champavère, Angélique; Gros, Edwige; Beurdeley, Pascale; Cruveiller, Stéphane; Tardy, Florence; Eloit, Marc

Desbrousses, Céline; Archer, Fabienne; Colin, Adélie; Bobet-Erny, Alexandra; Champavère, Angélique; Gros, Edwige; Beurdeley, Pascale; Cruveiller, Stéphane; Tardy, Florence; Eloit, Marc.

Afiliação

Desbrousses C; PathoQuest, Paris, France.
Archer F; University of Lyon, Université Claude Bernard Lyon1, INRAE, EPHE, IVPC, Viral Infections and Comparative Pathology, UMR754, F69007, Lyon, France.
Colin A; Université de Lyon, Anses Laboratoire de Lyon, VetAgro Sup, UMR Mycoplasmoses des Ruminants, F69364, Lyon, France.
Bobet-Erny A; University of Lyon, Université Claude Bernard Lyon1, INRAE, EPHE, IVPC, Viral Infections and Comparative Pathology, UMR754, F69007, Lyon, France.
Champavère A; University of Lyon, Université Claude Bernard Lyon1, INRAE, EPHE, IVPC, Viral Infections and Comparative Pathology, UMR754, F69007, Lyon, France.
Gros E; PathoQuest, Paris, France.
Beurdeley P; PathoQuest, Paris, France.
Cruveiller S; PathoQuest, Paris, France.
Tardy F; Université de Lyon, Anses Laboratoire de Lyon, VetAgro Sup, UMR Mycoplasmoses des Ruminants, F69364, Lyon, France.
Eloit M; PathoQuest, Paris, France; Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, 94704 Cedex, France; Pathogen Discovery Laboratory, Institut Pasteur, Paris, France. Electronic address: marc.eloit@pathoquest.com.

Biologicals ; 65: 18-24, 2020 May.

Article em En | MEDLINE | ID: mdl-32222272

Assuntos

Acholeplasma laidlawii/genética; Produtos Biológicos; Contaminação de Medicamentos; Sequenciamento de Nucleotídeos em Larga Escala/métodos; RNA Bacteriano/análise; Células A549; Humanos; Viabilidade Microbiana; Mycoplasma/genética; RNA-Seq; Análise de Sequência de RNA

Palavras-chave

4-Thiouridine; Biologicals; High throughput sequencing; Metagenomics; Mycoplasma; RNA-Seq; Sterility

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acholeplasma laidlawii / Produtos Biológicos / RNA Bacteriano / Contaminação de Medicamentos / Sequenciamento de Nucleotídeos em Larga Escala Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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