A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level.

Mair, Florian; Erickson, Jami R; Voillet, Valentin; Simoni, Yannick; Bi, Timothy; Tyznik, Aaron J; Martin, Jody; Gottardo, Raphael; Newell, Evan W; Prlic, Martin

Mair, Florian; Erickson, Jami R; Voillet, Valentin; Simoni, Yannick; Bi, Timothy; Tyznik, Aaron J; Martin, Jody; Gottardo, Raphael; Newell, Evan W; Prlic, Martin.

Afiliação

Mair F; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA.
Erickson JR; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA.
Voillet V; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA.
Simoni Y; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA.
Bi T; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA.
Tyznik AJ; BD Biosciences, La Jolla, CA 92037, USA.
Martin J; BD Biosciences, La Jolla, CA 92037, USA.
Gottardo R; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA; Fred Hutchinson Cancer Research Center, Public Health Sciences Division, Seattle, WA 98109, USA; Department of Statistics, University of Washington, Seattle, WA 98195, USA. Electronic address: rg
Newell EW; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA. Electronic address: enewell@fredhutch.org.
Prlic M; Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109, USA; Department of Global Health and Department of Immunology, University of Washington, Seattle, WA 98195, USA. Electronic address: mprlic@fredhutch.org.

Cell Rep ; 31(1): 107499, 2020 04 07.

Article em En | MEDLINE | ID: mdl-32268080

Assuntos

Sequenciamento de Nucleotídeos em Larga Escala/métodos; Análise de Sequência de RNA/métodos; Análise de Célula Única/métodos; Biologia Computacional/métodos; Epitopos/genética; Perfilação da Expressão Gênica/métodos; Humanos; Proteômica; RNA/genética; Software; Transcriptoma

Palavras-chave

AbSeq; One-SENSE; Rhapsody; barcoded antibody; high-dimensional cytometry; human immunology; multi-omic; single-cell RNA sequencing; targeted transcriptomics

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Análise de Sequência de RNA / Análise de Célula Única / Sequenciamento de Nucleotídeos em Larga Escala Limite: Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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