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Nuclear import of LIKE HETEROCHROMATIN PROTEIN1 is redundantly mediated by importins α-1, α-2 and α-3.
Chen, Chong; Kim, Daewon; Yun, Hee Rang; Lee, Yun Mi; Yogendra, Bordiya; Bo, Zhao; Kim, Hae Eun; Min, Jun Hong; Lee, Yong-Suk; Rim, Yeong Gil; Kim, Hyun Uk; Sung, Sibum; Heo, Jae Bok.
Afiliação
  • Chen C; Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Kim D; Systems and Synthetic Agrobiotech Center, Gyeongsang National University, Jinju, 660-701, Korea.
  • Yun HR; Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Lee YM; Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Yogendra B; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX, 78712, USA.
  • Bo Z; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX, 78712, USA.
  • Kim HE; Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Min JH; Department of Molecular Genetic Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Lee YS; Department of Biotechnology, Dong-A University, Busan, 604-714, Korea.
  • Rim YG; Systems and Synthetic Agrobiotech Center, Gyeongsang National University, Jinju, 660-701, Korea.
  • Kim HU; Department of Bioindustry and Bioresource Engineering, Sejong University, Seoul, 05006, Korea.
  • Sung S; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas, Austin, TX, 78712, USA.
  • Heo JB; International Scholar, Kyung-Hee University, Suwon, 17104, Korea.
Plant J ; 103(3): 1205-1214, 2020 08.
Article em En | MEDLINE | ID: mdl-32365248
LIKE HETEROCHROMATIN PROTEIN1 (LHP1) encodes the only plant homologue of the metazoan HETEROCHROMATIN PROTEIN1 (HP1) protein family. The LHP1 protein is necessary for proper epigenetic regulation of a range of developmental processes in plants. LHP1 is a transcriptional repressor of flowering-related genes, such as FLOWERING LOCUS T (FT), FLOWERING LOCUS C (FLC), AGAMOUS (AG) and APETALA 3 (AP3). We found that LHP1 interacts with importin α-1 (IMPα-1), importin α-2 (IMPα-2) and importin α-3 (IMPα-3) both in vitro and in vivo. A genetic approach revealed that triple mutation of impα-1, impα-2 and impα-3 resulted in Arabidopsis plants with a rapid flowering phenotype similar to that of plants with mutations in lhp1 due to the upregulation of FT expression. Nuclear targeting of LHP1 was severely impaired in the impα triple mutant, resulting in the de-repression of LHP1 target genes AG, AP3 and SHATTERPROOF 1 as well as FT. Therefore, the importin proteins IMPα-1, -2 and -3 are necessary for the nuclear import of LHP1.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Transporte Ativo do Núcleo Celular / Carioferinas / Alfa Carioferinas / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Transporte Ativo do Núcleo Celular / Carioferinas / Alfa Carioferinas / Proteínas de Arabidopsis Idioma: En Ano de publicação: 2020 Tipo de documento: Article