A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering.
Appl Microbiol Biotechnol
; 104(13): 6013-6022, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32367311
ABSTRACT
Directed evolution has become an important method to unleash the latent potential of enzymes to make them uniquely suited for human purposes. However, the need for a large reagent volume and sophisticated instrumentation hampers its broad implementation. In an attempt to address this problem, here we report a paper-based high-throughput screening approach that should find broad application in generating desired enzymes. As an example case, the dehalogenation reaction of the halohydrin dehalogenase was adopted for assay development. In addition to visual detection, quantitative measurements were performed by measuring the color intensity of an image that was photographed by a smartphone and processed using ImageJ free software. The proposed method was first validated using a gold standard method and then applied to mutagenesis library screening with reduced consumption of reagents (i.e., ≤ 10 µl per assay) and a shorter assay time. We identified two active mutants (P135A and G137A) with improved activities toward four tested substrates. The assay not only consumes less reagents but also eliminates the need for expensive instrumentation. The proposed method demonstrates the potential of paper-based whole-cell screening coupled with digital image colorimetry as a promising approach for the discovery of industrially important enzymes.Key Points⢠A frugal method was developed for directed enzyme evolution.⢠Mutagenesis libraries were successfully screened on a paper platform.⢠Smartphone imaging was efficiently used to measure enzyme activities.
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Base de dados:
MEDLINE
Assunto principal:
Papel
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Evolução Molecular Direcionada
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Ensaios de Triagem em Larga Escala
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article