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Effects of Biotin on survival, ensheathment, and ATP production by oligodendrocyte lineage cells in vitro.
Cui, Qiao-Ling; Lin, Yun Hsuan; Xu, Yu Kang T; Fernandes, Milton G F; Rao, Vijayaraghava T S; Kennedy, Timothy E; Antel, Jack.
Afiliação
  • Cui QL; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  • Lin YH; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  • Xu YKT; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  • Fernandes MGF; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  • Rao VTS; Medday Pharmaceuticals Inc., Boston, Massachusetts, United States of America.
  • Kennedy TE; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
  • Antel J; Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada.
PLoS One ; 15(5): e0233859, 2020.
Article em En | MEDLINE | ID: mdl-32470040
ABSTRACT
Mechanisms implicated in disease progression in multiple sclerosis include continued oligodendrocyte (OL)/myelin injury and failure of myelin repair. Underlying causes include metabolic stress with resultant energy deficiency. Biotin is a cofactor for carboxylases involved in ATP production that impact myelin production by promoting fatty acid synthesis. Here, we investigate the effects of high dose Biotin (MD1003) on the functional properties of post-natal rat derived oligodendrocyte progenitor cells (OPCs). A2B5 positive OPCs were assessed using an in vitro injury assay, culturing cells in either DFM (DMEM/F12+N1) or "stress media" (no glucose (NG)-DMEM), with Biotin added over a range from 2.5 to 250 µg/ml, and cell viability determined after 24 hrs. Biotin reduced the increase in OPC cell death in the NG condition. In nanofiber myelination assays, biotin increased the percentage of ensheathing cells, the number of ensheathed segments per cell, and length of ensheathed segments. In dispersed cell culture, Biotin also significantly increased ATP production, assessed using a Seahorse bio-analyzer. For most assays, the positive effects of Biotin were observed at the higher end of the dose-response analysis. We conclude that Biotin, in vitro, protects OL lineage cells from metabolic injury, enhances myelin-like ensheathment, and is associated with increased ATP production.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biotina / Trifosfato de Adenosina / Oligodendroglia / Linhagem da Célula Limite: Adult / Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biotina / Trifosfato de Adenosina / Oligodendroglia / Linhagem da Célula Limite: Adult / Animals / Humans Idioma: En Ano de publicação: 2020 Tipo de documento: Article