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Validation of the bag-mediated filtration system for environmental surveillance of poliovirus in Nairobi, Kenya.
Fagnant-Sperati, C S; Ren, Y; Zhou, N A; Komen, E; Mwangi, B; Hassan, J; Chepkurui, A; Nzunza, R; Nyangao, J; van Zyl, W B; Wolfaardt, M; Matsapola, P N; Ngwana, F B; Jeffries-Miles, S; Coulliette-Salmond, A; Peñaranda, S; Vega, E; Shirai, J H; Kossik, A L; Beck, N K; Boyle, D S; Burns, C C; Taylor, M B; Borus, P; Meschke, J S.
Afiliação
  • Fagnant-Sperati CS; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
  • Ren Y; Department of Biostatistics, University of Washington, Seattle, WA, USA.
  • Zhou NA; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
  • Komen E; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Mwangi B; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Hassan J; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Chepkurui A; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Nzunza R; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Nyangao J; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • van Zyl WB; Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
  • Wolfaardt M; Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
  • Matsapola PN; Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
  • Ngwana FB; Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
  • Jeffries-Miles S; Cherokee Nation Assurance a contracting agency to the Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Coulliette-Salmond A; Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Peñaranda S; Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Vega E; Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Shirai JH; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
  • Kossik AL; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
  • Beck NK; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
  • Boyle DS; PATH, Seattle, WA, USA.
  • Burns CC; Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Taylor MB; Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
  • Borus P; Centre for Viral Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Meschke JS; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
J Appl Microbiol ; 130(3): 971-981, 2021 Mar.
Article em En | MEDLINE | ID: mdl-32743931
ABSTRACT

AIMS:

This study compared the bag-mediated filtration system (BMFS) and standard WHO two-phase separation methods for poliovirus (PV) environmental surveillance, examined factors impacting PV detection and monitored Sabin-like (SL) PV type 2 presence with withdrawal of oral polio vaccine type 2 (OPV2) in April 2016. METHODS AND

RESULTS:

Environmental samples were collected in Nairobi, Kenya (Sept 2015-Feb 2017), concentrated via BMFS and two-phase separation methods, then assayed using the WHO PV isolation algorithm and intratypic differentiation diagnostic screening kit. SL1, SL2 and SL3 were detected at higher rates in BMFS than two-phase samples (P < 0·05). In BMFS samples, SL PV detection did not significantly differ with volume filtered, filtration time or filter shipment time (P > 0·05), while SL3 was detected less frequently with higher shipment temperatures (P = 0·027). SL2 was detected more frequently before OPV2 withdrawal in BMFS and two-phase samples (P < 1 × 10-5 ).

CONCLUSIONS:

Poliovirus was detected at higher rates with the BMFS, a method that includes a secondary concentration step, than using the standard WHO two-phase method. SL2 disappearance from the environment was commensurate with OPV2 withdrawal. SIGNIFICANCE AND IMPACT OF THE STUDY The BMFS offers comparable or improved PV detection under the conditions in this study, relative to the two-phase method.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Monitoramento Ambiental / Poliovirus / Filtração Tipo de estudo: Screening_studies Limite: Humans País/Região como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Monitoramento Ambiental / Poliovirus / Filtração Tipo de estudo: Screening_studies Limite: Humans País/Região como assunto: Africa Idioma: En Ano de publicação: 2021 Tipo de documento: Article