Plasma From Recovered COVID-19 Patients Inhibits Spike Protein Binding to ACE2 in a Microsphere-Based Inhibition Assay.
J Infect Dis
; 222(12): 1965-1973, 2020 11 13.
Article
em En
| MEDLINE
| ID: mdl-32798222
ABSTRACT
We present a microsphere-based flow cytometry assay that quantifies the ability of plasma to inhibit the binding of spike protein to angiotensin-converting enzyme 2. Plasma from 22 patients who had recovered from mild coronavirus disease 2019 (COVID-19) and expressed anti-spike protein trimer immunoglobulin G inhibited angiotensin-converting enzyme 2-spike protein binding to a greater degree than controls. The degree of inhibition was correlated with anti-spike protein immunoglobulin G levels, neutralizing titers in a pseudotyped lentiviral assay, and the presence of fever during illness. This inhibition assay may be broadly useful to quantify the functional antibody response of patients recovered from COVID-19 or vaccine recipients in a cell-free assay system.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Testes Sorológicos
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Anticorpos Neutralizantes
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Glicoproteína da Espícula de Coronavírus
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Enzima de Conversão de Angiotensina 2
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COVID-19
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Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article