Antibodies against the human cellular 3,3',5-triiodo-L-thyronine-binding protein (p 58).
FEBS Lett
; 230(1-2): 9-12, 1988 Mar 28.
Article
em En
| MEDLINE
| ID: mdl-3280347
ABSTRACT
High-titer antibodies against a cellular thyroid hormone-binding protein (Mr 58,000, p58) were developed by a special immunization method. To enhance immune responses, this method uses a boosting protocol in which repeated injections of small amounts of antigen are administered at 2-day intervals. Antibodies were detected 1 week after the last injection of antigen by ELISA, Western dot blotting and immunoprecipitation. The anti-p58 antibodies recognized p58 which is bound to the thyroid hormone. With the availability of anti-p58 antibodies, it has become possible to study cellular localization and function.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Hormônios Tireóideos
/
Proteínas de Transporte
/
Imunização
/
Proteínas de Membrana
/
Anticorpos
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article