Effect of nicotinamide supplementation in in vitro fertilization medium on bovine embryo development.
Mol Reprod Dev
; 87(10): 1070-1081, 2020 10.
Article
em En
| MEDLINE
| ID: mdl-32885880
ABSTRACT
Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of short-term NAM-treatment (3 and 5 h) during in vitro fertilization (IVF) on the development of bovine embryos. Treatment with 10 mM NAM for 3 h significantly increased the blastocyst formation but extending the treatment to 5 h did not enhance the benefits any further. Immunofluorescence analysis demonstrated that treatment with 10 mM NAM for 3 h decreased the expression of intracellular ROS, 8-oxo-7,8-dihydroguanine, caspase-3, and increased the expression of Sirt1, and incorporation of bromodeoxyuridine in one-cell stage embryos. Similarly, the level of H3K56ac significantly increased in the NAM-treated (3 and 5 h) one-cell stage embryos. Contrastingly, the treatment with 10 mM NAM for 5 h increased the caspase-9 level in blastocysts. Collectively, these findings suggest that NAM possesses antioxidant activity and supplementation of IVF medium with 10 mM NAM for 3 h improves the in vitro developmental competence of bovine embryos.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fertilização in vitro
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Niacinamida
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Desenvolvimento Embrionário
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article