Characterization of hydrogen sulfide toxicity to human corneal stromal fibroblasts.
Ann N Y Acad Sci
; 1480(1): 207-218, 2020 11.
Article
em En
| MEDLINE
| ID: mdl-32954509
ABSTRACT
Hydrogen sulfide gas (H2 S) is a chemical weapon and a common environmental pollutant. H2 S intoxication is lethal to humans and animals. H2 S contact to the eye can cause vision loss. However, the molecular mechanisms associated with H2 S toxicity to the cornea remain unclear, and no specific therapy exists to mitigate ocular damage from H2 S. Here, we report H2 S-induced cytotoxicity and the parameters contributing to the molecular mechanisms associated with corneal toxicity using primary human corneal stromal fibroblasts (hCSFs) in vitro. Sodium hydrosulfide (NaSH) was used as a source of H2 S, and the cytotoxicity of H2 S was determined by treating hCSF cells with varying concentrations of NaSH (0-10 mM) for 0-72 hours. Changes in cell proliferation, oxidative stress factors, and the expression of inflammatory and fibrotic genes were studied using standard commercial kits and qRT-PCR. NaSH exposure to hCSFs showed dose- and time-dependent cytotoxicity. The IC50 of NaSH was determined to be 5.35 mM. NaSH 5.35 mM exposure led to significantly decreased cytochrome c oxidase activity, increased ROS production, and increased expression of inflammatory and fibrotic genes in hCSF cells. H2 S/NaSH exposure alters normal mitochondrial function, oxidative stress, and inflammatory and fibrotic gene responses in corneal stromal fibroblasts in vitro.
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
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Estresse Oxidativo
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Substância Própria
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Citotoxinas
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Fibroblastos
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Sulfeto de Hidrogênio
Limite:
Humans
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article