Native detection of protein O-GlcNAcylation by gel electrophoresis.
Analyst
; 145(21): 6826-6830, 2020 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-33103664
ABSTRACT
O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of O-GlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetilglucosamina
/
Proteínas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article