Plasmid Acquisition Alters Vancomycin Susceptibility in Clostridioides difficile.
Gastroenterology
; 160(3): 941-945.e8, 2021 02.
Article
em En
| MEDLINE
| ID: mdl-33197449
ABSTRACT
The increasing incidence of primary and recurring Clostridioides difficile infections (CDI), which evade current treatment strategies, reflects the changing biology of C difficile. Here, we describe a putative plasmid-mediated mechanism potentially driving decreased sensitivity of C difficile to vancomycin treatment. We identified a broad host range transferable plasmid in a C difficile strain associated with lack of adequate response to vancomycin treatment. The transfer of this plasmid to a vancomycin-susceptible C difficile isolate decreased its susceptibility to vancomycin in vitro and resulted in more severe disease in a humanized mouse model. Our findings suggest plasmid acquisition in the gastrointestinal tract to be a possible mechanism underlying vancomycin treatment failure in patients with CDI, but further work is needed to characterize the mechanism by which plasmid genes determine vancomycin susceptibility in C difficile.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Vancomicina
/
Clostridioides difficile
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Infecções por Clostridium
/
Antibacterianos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article