Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing.
Sci Rep
; 10(1): 20496, 2020 11 24.
Article
em En
| MEDLINE
| ID: mdl-33235226
ABSTRACT
Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Herpesvirus Bovino 1
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Perfilação da Expressão Gênica
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Sequenciamento de Nucleotídeos em Larga Escala
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Transcriptoma
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article