Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing.

Moldován, Norbert; Torma, Gábor; Gulyás, Gábor; Hornyák, Ákos; Zádori, Zoltán; Jefferson, Victoria A; Csabai, Zsolt; Boldogkoi, Miklós; Tombácz, Dóra; Meyer, Florencia; Boldogkoi, Zsolt

Moldován, Norbert; Torma, Gábor; Gulyás, Gábor; Hornyák, Ákos; Zádori, Zoltán; Jefferson, Victoria A; Csabai, Zsolt; Boldogkoi, Miklós; Tombácz, Dóra; Meyer, Florencia; Boldogkoi, Zsolt.

Afiliação

Moldován N; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Torma G; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Gulyás G; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Hornyák Á; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungária krt. 21, 1143, Budapest, Hungary.
Zádori Z; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungária krt. 21, 1143, Budapest, Hungary.
Jefferson VA; Department of Biochemistry and Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 408 Dorman Hall, 32 Creelman St., Box 9655, Starkville, MS, 39762, USA.
Csabai Z; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Boldogkoi M; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Tombácz D; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary.
Meyer F; Department of Biochemistry and Molecular Biology, Entomology and Plant Pathology, Mississippi State University, 408 Dorman Hall, 32 Creelman St., Box 9655, Starkville, MS, 39762, USA.
Boldogkoi Z; Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., 6720, Szeged, Hungary. boldogkoi.zsolt@med.u-szeged.hu.

Sci Rep ; 10(1): 20496, 2020 11 24.

Article em En | MEDLINE | ID: mdl-33235226

ABSTRACT

ABSTRACT

Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.

Assuntos

Perfilação da Expressão Gênica; Herpesvirus Bovino 1/genética; Sequenciamento de Nucleotídeos em Larga Escala; Transcriptoma/genética; Processamento Alternativo/genética; Sequência de Bases; Linhagem Celular; Regulação Viral da Expressão Gênica; Genoma Viral; Íntrons/genética; Cinética; Anotação de Sequência Molecular; Peptídeos/metabolismo; Regiões Promotoras Genéticas/genética; Isoformas de Proteínas/genética; Isoformas de Proteínas/metabolismo; RNA Mensageiro/genética; RNA Mensageiro/metabolismo; Fatores de Tempo; Sítio de Iniciação de Transcrição; Transcrição Gênica

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Herpesvirus Bovino 1 / Perfilação da Expressão Gênica / Sequenciamento de Nucleotídeos em Larga Escala / Transcriptoma Idioma: En Ano de publicação: 2020 Tipo de documento: Article

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