Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T<sub>1</sub> generation.

Adachi, Kohei; Hirose, Aya; Kanazashi, Yuhei; Hibara, Miki; Hirata, Toshiyuki; Mikami, Masafumi; Endo, Masaki; Hirose, Sakiko; Maruyama, Nobuyuki; Ishimoto, Masao; Abe, Jun; Yamada, Tetsuya

Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T₁ generation.

Adachi, Kohei; Hirose, Aya; Kanazashi, Yuhei; Hibara, Miki; Hirata, Toshiyuki; Mikami, Masafumi; Endo, Masaki; Hirose, Sakiko; Maruyama, Nobuyuki; Ishimoto, Masao; Abe, Jun; Yamada, Tetsuya.

Afiliação

Adachi K; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan.
Hirose A; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan.
Kanazashi Y; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan.
Hibara M; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan.
Hirata T; Field Science Center Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kita-ku, Sapporo, Hokkaido, 060-0811, Japan.
Mikami M; Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agricultural and Food Research Organization, 1-2, Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
Endo M; Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agricultural and Food Research Organization, 1-2, Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
Hirose S; Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agricultural and Food Research Organization, 1-2, Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
Maruyama N; Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan.
Ishimoto M; Plant Genome Engineering Research Unit, Institute of Agrobiological Sciences, National Agricultural and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
Abe J; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan.
Yamada T; Graduate School of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido, 060-8589, Japan. tetsuyay@res.agr.hokudai.ac.jp.

Transgenic Res ; 30(1): 77-89, 2021 02.

Article em En | MEDLINE | ID: mdl-33386504

Assuntos

Antígenos de Plantas/genética; Sistemas CRISPR-Cas/genética; Glycine max/genética; Proteínas de Soja/genética; Transgenes/genética; Antígenos de Plantas/efeitos adversos; Antígenos de Plantas/imunologia; Biolística; Produtos Agrícolas/genética; Edição de Genes; Genoma de Planta; Humanos; Mutagênese Sítio-Dirigida; Mutação/genética; Melhoramento Vegetal; Plantas Geneticamente Modificadas/genética; Plantas Geneticamente Modificadas/crescimento & desenvolvimento; Plantas Geneticamente Modificadas/imunologia; Proteínas de Soja/efeitos adversos; Proteínas de Soja/imunologia; Glycine max/crescimento & desenvolvimento; Glycine max/imunologia; Transgenes/imunologia

Palavras-chave

CRISPR/Cas9; Glycine max; Nonsense mRNA decay; Null-segregant; Transgenic embryo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Glycine max / Transgenes / Proteínas de Soja / Antígenos de Plantas / Sistemas CRISPR-Cas Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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