Docking domain-mediated subunit interactions in natural product megasynth(et)ases.
J Ind Microbiol Biotechnol
; 48(3-4)2021 Jun 04.
Article
em En
| MEDLINE
| ID: mdl-33640957
ABSTRACT
Polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) multienzymes produce numerous high value metabolites. The protein subunits which constitute these megasynth(et)ases must undergo ordered self-assembly to ensure correct organisation of catalytic domains for the biosynthesis of a given natural product. Short amino acid regions at the N- and C-termini of each subunit, termed docking domains (DDs), often occur in complementary pairs, which interact to facilitate substrate transfer and maintain pathway fidelity. This review details all structurally characterised examples of NRPS and PKS DDs to date and summarises efforts to utilise DDs for the engineering of biosynthetic pathways.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Sintases
/
Produtos Biológicos
/
Policetídeo Sintases
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article