Single-cell measurement of plasmid copy number and promoter activity.
Nat Commun
; 12(1): 1475, 2021 03 05.
Article
em En
| MEDLINE
| ID: mdl-33674569
ABSTRACT
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Bactérias
/
Variações do Número de Cópias de DNA
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article