Structure of the dihydrolipoamide succinyltransferase (E2) component of the human alpha-ketoglutarate dehydrogenase complex (hKGDHc) revealed by cryo-EM and cross-linking mass spectrometry: Implications for the overall hKGDHc structure.

Nagy, Balint; Polak, Martin; Ozohanics, Oliver; Zambo, Zsofia; Szabo, Eszter; Hubert, Agnes; Jordan, Frank; Novacek, Jirí; Adam-Vizi, Vera; Ambrus, Attila

Nagy, Balint; Polak, Martin; Ozohanics, Oliver; Zambo, Zsofia; Szabo, Eszter; Hubert, Agnes; Jordan, Frank; Novacek, Jirí; Adam-Vizi, Vera; Ambrus, Attila.

Afiliação

Nagy B; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Polak M; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Ozohanics O; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Zambo Z; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Szabo E; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Hubert A; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Jordan F; Department of Chemistry, Rutgers, The State University of New Jersey, Newark, NJ, USA.
Novacek J; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Adam-Vizi V; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Ambrus A; Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary. Electronic address: ambrus.attila@med.semmelweis-univ.hu.

Biochim Biophys Acta Gen Subj ; 1865(6): 129889, 2021 06.

Article em En | MEDLINE | ID: mdl-33684457

Assuntos

Aciltransferases/química; Aciltransferases/metabolismo; Reagentes de Ligações Cruzadas/química; Microscopia Crioeletrônica/métodos; Complexo Cetoglutarato Desidrogenase/química; Complexo Cetoglutarato Desidrogenase/metabolismo; Espectrometria de Massas/métodos; Acil Coenzima A/metabolismo; Humanos; Ácidos Cetoglutáricos/metabolismo; Modelos Moleculares; NAD/metabolismo; Conformação Proteica

Palavras-chave

2-Oxoglutarate dehydrogenase complex; Cross-linking mass spectrometry; Cryo-electron microscopy; Dihydrolipoamide succinyltransferase; α-Ketoglutarate dehydrogenase complex

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Aciltransferases / Microscopia Crioeletrônica / Reagentes de Ligações Cruzadas / Complexo Cetoglutarato Desidrogenase Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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