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Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity.
Shirai, Sahoko; Uemura, Ryota; Maeda, Megumi; Kajiura, Hiroyuki; Misaki, Ryo; Fujiyama, Kazuhito; Kimura, Yoshinobu.
Afiliação
  • Shirai S; Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
  • Uemura R; Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
  • Maeda M; Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
  • Kajiura H; International Center for Biotechnology, Osaka University, Osaka, Japan.
  • Misaki R; International Center for Biotechnology, Osaka University, Osaka, Japan.
  • Fujiyama K; International Center for Biotechnology, Osaka University, Osaka, Japan.
  • Kimura Y; Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
Biosci Biotechnol Biochem ; 85(6): 1460-1463, 2021 May 25.
Article em En | MEDLINE | ID: mdl-33724383
Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Citosol / Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Citosol / Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase Idioma: En Ano de publicação: 2021 Tipo de documento: Article