Generation of Patient-Derived Colorectal Cancer Organoids for RAS Studies.
Methods Mol Biol
; 2262: 349-360, 2021.
Article
em En
| MEDLINE
| ID: mdl-33977489
ABSTRACT
Human cell line models have been widely used for testing of novel anticancer compounds and for predicting clinical response to monotherapies and combinatorial therapies. For many years, standard monolayer culture conditions were used as gold standard, only surpassed by in vivo testing of mouse models. Recently, the incorporation of three-dimensional culture has been shown to further improve predictive compound testing. In view of the renewed interest in anti-RAS cancer therapy, we provide a protocol for establishing colorectal cancer organoids which are characterized by a high prevalence of KRAS mutations.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Órgãos
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Neoplasias Colorretais
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Organoides
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Biomarcadores Tumorais
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Proteínas ras
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Mutação
Tipo de estudo:
Guideline
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article