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The Stimulating Effect of Rosmarinic Acid and Extracts from Rosemary and Lemon Balm on Collagen Type I Biosynthesis in Osteogenesis Imperfecta Type I Skin Fibroblasts.
Sutkowska, Joanna; Hupert, Natalia; Gawron, Katarzyna; Strawa, Jakub W; Tomczyk, Michal; Forlino, Antonella; Galicka, Anna.
Afiliação
  • Sutkowska J; Department of Medical Chemistry, Medical University of Bialystok, ul. Mickiewicza 2A, 15-222 Bialystok, Poland.
  • Hupert N; Department of Molecular Biology and Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, Poland.
  • Gawron K; Department of Molecular Biology and Genetics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, 40-752 Katowice, Poland.
  • Strawa JW; Department of Pharmacognosy, Medical University of Bialystok, ul. Mickiewicza 2A, 15-230 Bialystok, Poland.
  • Tomczyk M; Department of Pharmacognosy, Medical University of Bialystok, ul. Mickiewicza 2A, 15-230 Bialystok, Poland.
  • Forlino A; Department of Molecular Medicine, Biochemistry Unit, University of Pavia, 27100 Pavia, Italy.
  • Galicka A; Department of Medical Chemistry, Medical University of Bialystok, ul. Mickiewicza 2A, 15-222 Bialystok, Poland.
Pharmaceutics ; 13(7)2021 Jun 23.
Article em En | MEDLINE | ID: mdl-34201872
ABSTRACT
Rosemary extract (RE) and lemon balm extract (LBE) attract particular attention of pharmacists due to their high therapeutic potential. Osteogenesis imperfecta (OI) type I is a heritable disease caused by mutations in type I collagen and characterized by its reduced amount. The aim of the study was to evaluate the effect of the extracts and rosmarinic acid (RA) on collagen type I level in OI skin fibroblasts. Phytochemical analysis of RE and LBE was carried out by liquid chromatography-photodiode array detection-mass spectrometry. The expression of collagen type I at transcript and protein levels was analyzed by qPCR, ELISA, SDS-urea PAGE, and Western blot. In OI patient's fibroblasts the exposure to the extracts (0.1-100 µg/mL) and RA (0.1-100 µM) significantly increased collagen type I and the best results were obtained with 0.1-10 µM RA and 0.1-10 µg/mL of the extracts. LBE showed a greater stimulating effect than RE, likely due to a higher RA content. Moreover, collagen type III expression and matrix metalloproteinase (MMP-1, -2, -9) activity remained unchanged or decreased. The obtained data support the clinical potential of RA-rich extracts and RA itself in modulating the quantitative defect of type I collagen in type I OI.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article