Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide.

Rigi, Garshasb; Rostami, Amin; Ghomi, Habib; Ahmadian, Gholamreza; Mirbagheri, Vasiqe Sadat; Jeiranikhameneh, Meisam; Vahed, Majid; Rahimi, Sahel

Rigi, Garshasb; Rostami, Amin; Ghomi, Habib; Ahmadian, Gholamreza; Mirbagheri, Vasiqe Sadat; Jeiranikhameneh, Meisam; Vahed, Majid; Rahimi, Sahel.

Afiliação

Rigi G; Department of Genetics, Faculty of Basic Science, Shahrekord University, P. O. Box 115, Shahrekord, 881 863 4141, Iran.
Rostami A; Department of Industrial Biotechnology, Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran.
Ghomi H; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Ahmadian G; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Mirbagheri VS; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. ahmadian@nigeb.ac.ir.
Jeiranikhameneh M; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Vahed M; Fisheries products processing group, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Rahimi S; Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

BMC Biotechnol ; 21(1): 51, 2021 08 16.

Article em En | MEDLINE | ID: mdl-34399745

Assuntos

Escherichia coli/genética; Expressão Gênica; Hormônio do Crescimento Humano/genética; Hormônio do Crescimento Humano/isolamento & purificação; Proteína Estafilocócica A/genética; Meios de Cultura/química; Meios de Cultura/metabolismo; Escherichia coli/metabolismo; Hormônio do Crescimento Humano/metabolismo; Humanos; Sinais Direcionadores de Proteínas; Transporte Proteico; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Proteínas Recombinantes/metabolismo; Proteína Estafilocócica A/metabolismo

Palavras-chave

Recombinant hGH; Sec pathway; Secretion; SpA signal peptide

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteína Estafilocócica A / Expressão Gênica / Hormônio do Crescimento Humano / Escherichia coli Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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