Boerhaavia diffusa Extract Acts as a Specific Antituberculosis Agent in Vitro Against Mycobacterium tuberculosis H37Rv Infection.
Dokl Biochem Biophys
; 499(1): 266-272, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-34426925
ABSTRACT
The present study investigated Boerhaavia diffusa extract against Mycobacterium tuberculosis H37Rv (M.tb) infection in vitro and explored the underlying mechanism. The study demonstrated that Boerhaavia diffusa extract significantly (p < 0.05) reduced RAW 264.7 and A549 cell viability in concentration dependent manner. In BEAS-2B, NuLi-1 cells and splenocytes no significant (p > 0.05) reduction in viability was observed on treatment with 2.5 to 20 mg/L concentrations of Boerhaavia diffusa. The M. tbinduced increase in TNFα expression was significantly (p < 0.05) reversed by Boerhaavia diffusa treatment in RAW 264.7 and BEAS-2B cells. Moreover, Boerhaavia diffusa treatment significantly (p < 0.05) inhibited M.tbinduced increase in IL-6 and IL1ß expression in RAW 264.7 and BEAS-2B cells. Boerhaavia diffusa treatment of RAW 264.7 and BEAS-2B cells significantly (p < 0.05) reversed M.tbinduced increase in iNOS and COX2 expression. Additionally, in Boerhaavia diffusa treated cells M.tbinduced increase in NO release was significantly (p < 0.05) reduced compared to untreated cells. In summary, Boerhaavia diffusa treatment inhibits pro-inflammatory cytokine production, NO release and regulate immunomodulatory mediators in M.tbinfected RAW 264.7 and BEAS-2B cells. Therefore, Boerhaavia diffusa may be developed as a therapeutic agent for treatment of M.tbinfection.
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Nyctaginaceae
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Mycobacterium tuberculosis
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Antituberculosos
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article