Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas.

Patinios, Constantinos; Creutzburg, Sjoerd C A; Arifah, Adini Q; Adiego-Pérez, Belén; Gyimah, Evans A; Ingham, Colin J; Kengen, Servé W M; van der Oost, John; Staals, Raymond H J

Patinios, Constantinos; Creutzburg, Sjoerd C A; Arifah, Adini Q; Adiego-Pérez, Belén; Gyimah, Evans A; Ingham, Colin J; Kengen, Servé W M; van der Oost, John; Staals, Raymond H J.

Afiliação

Patinios C; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Creutzburg SCA; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Arifah AQ; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Adiego-Pérez B; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Gyimah EA; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Ingham CJ; Hoekmine Besloten Vennootschap, Kenniscentrum Technologie en Innovatie, Hogeschool Utrecht, 3584 CS, Utrecht, The Netherlands.
Kengen SWM; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
van der Oost J; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Staals RHJ; Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.

Nucleic Acids Res ; 49(19): 11392-11404, 2021 11 08.

Article em En | MEDLINE | ID: mdl-34614191

Assuntos

Escherichia coli/genética; Flavobacterium/genética; Edição de Genes/métodos; Genoma Bacteriano; Pseudomonas putida/genética; RNA Bacteriano/genética; Pareamento de Bases; Sequência de Bases; Sistemas CRISPR-Cas; Escherichia coli/metabolismo; Flavobacterium/metabolismo; Técnicas de Inativação de Genes/métodos; Recombinação Homóloga; Íntrons; Conformação de Ácido Nucleico; Pseudomonas putida/metabolismo; Splicing de RNA; RNA Bacteriano/metabolismo; Riboswitch

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Bacteriano / Flavobacterium / Pseudomonas putida / Genoma Bacteriano / Escherichia coli / Edição de Genes Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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