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Proof of concept of a multimodal intravital molecular imaging system for tumour transpathology investigation.
Liu, Zhen; Cheng, Tao; Düwel, Stephan; Jian, Ziying; Topping, Geoffrey J; Steiger, Katja; Wang, Qian; Braren, Rickmer; Reder, Sybille; Mittelhäuser, Markus; Hundshammer, Christian; Feuerecker, Benedikt; Huang, Sung-Cheng; Schwaiger, Markus; Schilling, Franz; Ziegler, Sibylle I; Shi, Kuangyu.
Afiliação
  • Liu Z; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Cheng T; Institute of Biomedical Engineering, Shenzhen Bay Laboratory, Shenzhen, China.
  • Düwel S; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Jian Z; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Topping GJ; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Steiger K; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Wang Q; Department of Pathology, School of Medicine, Technische Universität München, Munich, Germany.
  • Braren R; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Reder S; Department of Radiology, Technische Universität München, Munich, Germany.
  • Mittelhäuser M; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Hundshammer C; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Feuerecker B; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Huang SC; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Schwaiger M; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Schilling F; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Ziegler SI; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
  • Shi K; Department of Nuclear Medicine, School of Medicine, Technische Universität München, Munich, Germany.
Eur J Nucl Med Mol Imaging ; 49(4): 1157-1165, 2022 03.
Article em En | MEDLINE | ID: mdl-34651225
ABSTRACT

BACKGROUND:

Transpathology highlights the interpretation of the underlying physiology behind molecular imaging. However, it remains challenging due to the discrepancies between in vivo and in vitro measurements and difficulties of precise co-registration between trans-scaled images. This study aims to develop a multimodal intravital molecular imaging (MIMI) system as a tool for in vivo tumour transpathology investigation.

METHODS:

The proposed MIMI system integrates high-resolution positron imaging, magnetic resonance imaging (MRI) and microscopic imaging on a dorsal skin window chamber on an athymic nude rat. The window chamber frame was designed to be compatible with multimodal imaging and its fiducial markers were customized for precise physical alignment among modalities. The co-registration accuracy was evaluated based on phantoms with thin catheters. For proof of concept, tumour models of the human colorectal adenocarcinoma cell line HT-29 were imaged. The tissue within the window chamber was sectioned, fixed and haematoxylin-eosin (HE) stained for comparison with multimodal in vivo imaging.

RESULTS:

The final MIMI system had a maximum field of view (FOV) of 18 mm × 18 mm. Using the fiducial markers and the tubing phantom, the co-registration errors are 0.18 ± 0.27 mm between MRI and positron imaging, 0.19 ± 0.22 mm between positron imaging and microscopic imaging and 0.15 ± 0.27 mm between MRI and microscopic imaging. A pilot test demonstrated that the MIMI system provides an integrative visualization of the tumour anatomy, vasculatures and metabolism of the in vivo tumour microenvironment, which was consistent with ex vivo pathology.

CONCLUSIONS:

The established multimodal intravital imaging system provided a co-registered in vivo platform for trans-scale and transparent investigation of the underlying pathology behind imaging, which has the potential to enhance the translation of molecular imaging.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento por Ressonância Magnética / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento por Ressonância Magnética / Neoplasias Limite: Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article