Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces.
ACS Photonics
; 8(10): 3111-3118, 2021 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-34692901
ABSTRACT
Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterization of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable when localization precision and imaging speed are paramount due to the in principle infinite photon budget. Here, we show that micromirror-based total internal reflection dark field microscopy enables background suppression previously only reported for interferometric scattering microscopy, resulting in nanometer localization precision at 6 µs exposure time for 20 nm gold nanoparticles with a 25 × 25 µm2 field of view. We demonstrate the capabilities of our implementation by characterizing sub-nanometer deterministic flows of 20 nm gold nanoparticles at liquid-liquid interfaces. Our results approach the optimal combination of background suppression, localization precision, and temporal resolution achievable with pure scattering-based imaging and tracking of nanoparticles at interfaces.
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MEDLINE
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En
Ano de publicação:
2021
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Article