Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search.

Yang, Mengyi; Sun, Ruirui; Deng, Pujuan; Yang, Yuzhuo; Wang, Wenjuan; Liu, Jun-Jie Gogo; Chen, Chunlai.

Afiliação

Yang M; School of Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Tsinghua University Beijing China chunlai@mail.tsinghua.edu.cn.
Sun R; Laboratory of Nutrition and Development, Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing Pediatric Research Institutue, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health Beijing 100045 China.
Deng P; School of Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Tsinghua University Beijing China chunlai@mail.tsinghua.edu.cn.
Yang Y; School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Tsinghua University Beijing China junjiegogoliu@tsinghua.edu.cn.
Wang W; School of Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Beijing Frontier Research Center for Biological Structure, Tsinghua University Beijing China chunlai@mail.tsinghua.edu.cn.
Liu JG; School of Life Sciences, Technology Center for Protein Sciences, Tsinghua University Beijing China.
Chen C; School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Tsinghua University Beijing China junjiegogoliu@tsinghua.edu.cn.

Chem Sci ; 12(38): 12776-12784, 2021 Oct 06.

Article em En | MEDLINE | ID: mdl-34703564

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2021 Tipo de documento: Article